AMMRF -

High-Throughput Cryo-TEM Facility

Contact

Centre for Microscopy and Microanalysis; The University of Queensland
Garry Morgan, Infrastructure Support Engineer/Scientist
Tel: 07 3346 2935
Email: g.morgan@imb.uq.edu.au

The facility is built around a Tecnai G2 F30 TWIN 300 keV Field Emission Gun (FEG) transmission electron microscope. Incorporating a range of purpose built holders that can be cooled effectively to liquid nitrogen temperatures, the instrument is designed to collect high-resolution data on biological active molecules and organelles. This instrument incorporates the latest in digital capture in the form of a bottom-mounted 4k by 4k Gatan Utrascan 4000 SP digital CCD camera.

The instrument also includes an embedded Gatan Imaging Filter (GIF) system, which will allow imaging information to be obtained based on the elemental distribution in the specimen. This microscope is supported by a range of the latest in sample preparation equipment specifically designed to ensure that biological specimens can be prepared with minimum interference or damage prior to examination using electron microscopy.

Capabilities & configuration
  • Whole cell tomography
  • Single particle macromolecular imaging
  • Protein crystallography
  • New 300 kV FEG instrument
  • Electron optical column optimised for biological microscopy with advanced cryo-transfer system
  • Specialised cold-trap
  • Automation for remote use
  • High-resolution digital image acquisition system
High-troughput cryo-TEM stage
Example image

Investment in the latest technologies (e.g., ultra-high-resolution CCD cameras, specialist cryo-holders etc.) will create a world-leading platform for high-throughput molecular biology.

Three-dimensional map of the
archaeal thermosome holoenzyme
.

TOP
Flagship instruments

Cameca IMS 1280 Ion Probe
Cameca NanoSIMS 50 Ion Probe
The University of Western Australia

High-throughput cryo-TEM facility
The University of Queensland

Imago Local Electrode Atom Probes
The University of Sydney

FEI Nova NanoLab 200 DualBeam FIB
The University of New South Wales

FEI Helios NanoLab DualBeam FIB
The University of Adelaide; South Australian Regional Facility (SARF)

High-resolution SEM microanalysis facility
The University of New South Wales

PHI TRIFT V nanoToF ToF-SIMS
University of South Australia; South Australian Regional Facility (SARF)